Methods of ion channel reconstitution in this section i will describe methods for the formation of artificial lipid bilayers and the incorporation of ion channels into these membranes. Ion channel reconstitution involves the incorporation of intact membrane vesicles containing proteins or of water soluble channels into an artificial planar lipid bilayer figure 3 the most common configuration used for planar bilayer recordings is the open chamber configuration. Ion channels have gained increased interest as therapeutic targets over recent years since a growing number of human and animal diseases have been attributed to defects in ion channel function. In this chapter a detailed protocol is given for ion channel reconstitution in the two most used model membranes planar bilayers and liposomes in the planar bilayer section methods are described for the expression of ion channels in xenopus laevis oocytes the isolation of their membranes the . The ion currents carried by single channels can be measured by patch clamp recording or by lipid bilayer reconstitution in the patch clamp developed by neher and co workers 1 a patch of cell membrane small enough to give good signal resolution is electrically isolated with a glass micropipette
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